Which tests should be ordered for a 44-year-old woman with regular menstrual cycles who is considering hormone replacement therapy? | Rounds Which tests should be ordered for a 44-year-old woman with regular menstrual cycles who is considering hormone replacement therapy? | Rounds
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Which tests should be ordered for a 44-year-old woman with regular menstrual cycles who is considering hormone replacement therapy?

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Last updated: July 14, 2026 · View editorial policy

Pre-HT Assessment Tests for Menopausal Hormone Therapy

Menopausal hormone therapy (MHT) decisions should be based on an assessment of baseline cardiovascular disease risk and on age-appropriate cancer screening status. [1]

Routine hormone-level testing is not recommended for initiating MHT for menopausal symptoms. [2]

Baseline Clinical Tests

  • Blood pressure measurement is recommended as part of the baseline evaluation for MHT risk assessment. [3]
  • Body weight and body mass index assessment are recommended as part of the baseline evaluation for health risk assessment. [3]

Baseline Laboratory Tests

  • Lipid testing is recommended as part of baseline laboratory evaluation for cardiovascular risk assessment. [3]
  • Fasting blood glucose testing is recommended as part of baseline laboratory evaluation for metabolic risk assessment. [3]
  • Liver function testing is recommended as part of baseline laboratory evaluation before MHT. [3]
  • Kidney function testing is recommended as part of baseline laboratory evaluation before MHT. [3]
  • Complete blood count testing is recommended as part of baseline laboratory evaluation before MHT. [3]

Pregnancy Exclusion Testing Before Systemic Estrogen

  • For women who are not definitively postmenopausal and who still have uterine bleeding, pregnancy should be addressed before starting systemic MHT. [4]
  • Unwanted pregnancy prevention should be considered when initiating hormone therapy prior to final menstrual period. [4]

Cancer Screening–Related Tests

  • Breast cancer screening with screening mammography should be ensured based on age-appropriate U.S. preventive screening recommendations. [5]
  • Postmenopausal bleeding should be evaluated promptly for endometrial pathology when it occurs during hormone therapy. [4]

Approach to Cervical Cancer Screening

  • Cervical cancer screening should be kept up to date according to routine age-based screening recommendations while considering MHT. [3]

Hormone Level Testing

  • FSH, estradiol, and other hormone blood tests are not recommended solely to determine whether MHT should be started for menopausal symptom treatment. [2]

Sources

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