When should anti‑D immunoglobulin (Rhogam) be administered to an Rh‑negative mother after delivery of an Rh‑positive infant? | Rounds When should anti‑D immunoglobulin (Rhogam) be administered to an Rh‑negative mother after delivery of an Rh‑positive infant? | Rounds
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When should anti‑D immunoglobulin (Rhogam) be administered to an Rh‑negative mother after delivery of an Rh‑positive infant?

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Last updated: July 14, 2026 · View editorial policy

Postpartum Anti-D Immunoglobulin for Rh-Negative Mothers After Rh-Positive Delivery

Anti-D (Rho(D) immune globulin) should be administered to an Rh-negative, nonsensitized mother following delivery of an Rh-positive infant within 72 hours of birth. [1]

Timing After Delivery

  • Anti-D should be administered within 72 hours of delivery. [1], [3]

Indications After Birth

Anti-D is indicated when an Rh-negative, nonsensitized mother delivers an Rh-positive infant. [1]

Standard Postpartum Dose

  • Anti-D 300 microg (intramuscular or intravenous) is recommended postpartum. [1], [3]

Additional Dosing for Fetomaternal Hemorrhage

  • Additional Anti-D should be administered when fetomaternal hemorrhage (FMH) exceeds the dose coverage. [1]
  • SOGC guidance specifies additional Anti-D when FMH is greater than 15 mL of fetal red blood cells, based on quantitative FMH testing. [1]
  • An alternative dosing approach uses Anti-D 120 microg postpartum with FMH testing to guide additional dosing when FMH exceeds 6 mL of fetal red blood cells. [1]

Common Clinical Constraints

  • Routine postpartum testing for FMH is supported by limited evidence because cost-benefit data are insufficient. [1]

When Anti-D Is Not Given Within the Usual Window

  • If Anti-D is not administered within 72 hours, Anti-D should be given as soon as possible for up to 28 days after delivery or other potentially sensitizing event. [1]

Product Label–Consistent Timing

  • Full-dose Anti-D (1500 IU, equivalent to 300 microg) is labeled for prevention of Rh hemolytic disease by administration to an Rh(D)-negative mother within 72 hours after birth of an Rh(D)-positive infant (when indicated criteria are met). [3]

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